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Presently, choices for disposal of livestock carcasses includes rendering, burial, landfills, composting and incineration. In high livestock density areas (areas where there may be several large feedlots or dairies), animal mortalities and tissues discarded from the slaughter of animals are rendered into usable materials such as lard or tallow. Some of these products are utilized in the manufacturing of wet and dry pet foods. In February 2018, pentobarbital contaminated pet food was alleged to result in the death of a dog [27]. Subsequent testing by the Food and Drug Administration (FDA) (United States) confirmed the presence of pentobarbital in a tallow ingredient used in the dog food prompting an immediate nationwide recall of all potentially affected pet food products [28]. Although death or serious illness of animals consuming pentobarbital-contaminated pet food are unlikely, studies confirm that the pentobarbital will persist through the rendering process [29]. In a study designed to assess the distribution and fate of pentobarbital in rendered material from euthanized animals, researchers found it equally distributed between the meat and bone meal and tallow fractions [29].

Burial is a common method of carcass disposal and regulated by local laws and ordinances as to the number of pounds of animal carcass per acre per year that may be buried. It is best suited to geographic areas where the water table is deep and the soil is non-porous. Most regions have soil survey maps that can be used to determine the suitability of a specific site for carcass burial considering water table depth and soil porosity. Carcasses from euthanized animals can leach pentobarbital into the surrounding soil and ground water [30].

Landfills are a convenient and potentially cost effective way to dispose of animal mortalities where landfill operators will accept animal carcasses. Modern landfills are designed to protect the environment offering not only containment, but also a barrier to prevent wildlife access. Nonetheless, testing of water supplies near landfills has found detectable levels of pentobarbital up to 22 years following the time in which wastes were received [30].

Composting is a relatively inexpensive and environmentally sound approach to carcass disposal in conditions where compost piles can be properly managed. A recent study of horses euthanized with pentobarbital found that leachate from the decomposing carcass penetrated through the compost bed and into the soil beneath. Researchers concluded that it was not only important to provide a protective physical barrier to reduce the risk to scavenging by wildlife, but also recognize the risks of surface, groundwater and soil contamination [31].

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In high doses, pentobarbital causes death by respiratory arrest. It is used for veterinary euthanasia and is used by some U.S. states and the U.S. federal government for executions of convicted criminals by lethal injection.[2] In some countries and states, it is also used for physician-assisted suicide.[3][4]

Typical applications for pentobarbital are sedative, short term hypnotic, preanesthetic, insomnia treatment, and control of convulsions in emergencies.[1] Abbott Pharmaceutical discontinued manufacture of their Nembutal brand of Pentobarbital capsules in 1999, largely replaced by the benzodiazepine family of drugs. They were available as 30, 50, and 100 mg capsules of yellow, white-orange, and yellow colors, respectively.[5]

It is taken by mouth for physician-assisted death in the United States states of Oregon, Washington, Vermont, and California (as of January 2016).[10][11] The oral dosage of pentobarbital indicated for physician-assisted suicide in Oregon is typically 10 g of liquid.[10][11]

In Switzerland, sodium pentobarbital is administered to the patient intravenously. Once administered, sleep is induced within 30 seconds, and the heart stops beating within 3 minutes.[12] Oral administration is also used. A Swiss pharmacist reported in 2022 that the dose for assisted suicide had been raised to 15 grams because with lower doses in some cases death was preceded by a coma of up to 10 hours.[13]

Pentobarbital has been used or considered as a substitute for the barbiturate sodium thiopental used for capital punishment by lethal injection in the United States when that drug became unavailable.[14] In 2011 the U.S. manufacturer of sodium thiopental stopped production, and importation of the drug proved impossible. Pentobarbital was used in an U.S. execution for the first time in December 2010 in Oklahoma, as part of a three-drug protocol.[14] In March 2011 pentobarbital was used for the first time as the sole drug in a U.S. execution, in Ohio. Since then several states as well as the federal government have used pentobarbital for lethal injections; some use three-drug protocols and others use pentobarbital alone.

Texas began using the single-drug pentobarbital protocol for executing death-row inmates on July 18, 2012,[16] because of a shortage of pancuronium bromide, a muscle paralytic previously used as one component of a three-drug cocktail.[16] In October 2013, Missouri changed its protocol to allow for pentobarbital from a compounding pharmacy to be used in a lethal dose for executions.[17] It was first used in November 2013.[18][19]

Pentobarbital is synthesized by methods analogous to that of amobarbital, the only difference being that the alkylation of α-ethylmalonic ester is carried out with 2-bromopentane in place of 1-bromo-3-methylbutane to give pentobarbital.[24][25][26]

Pentobarbital can occur as a free acid, but is usually formulated as the sodium salt, pentobarbital sodium. The free acid is only slightly soluble in water and in ethanol[27][28] while the sodium salt shows better solubility.

Abbott discontinued their Nembutal brand of pentobarbital capsules in 1999, largely replaced by the benzodiazepine family of drugs. Abbott's Nembutal, known on the streets as "yellow jackets", was widely abused.[30][31]

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